Journal: Vaccines

Article Title: Construction and Evaluation of the Immunogenicity and Protective Efficacy of Recombinant Replication-Deficient Human Adenovirus-5 Expressing Genotype VII Newcastle Disease Virus F Protein and Infectious Bursal Disease Virus VP2 Protein

doi: 10.3390/vaccines11061051

Figure Lengend Snippet: Comparison of cellular immunity in different groups: ( A ) Statistical analysis of the peripheral blood lymphocyte stimulation index in the ND groups. Under ConA stimulation, the SI values of peripheral blood lymphocytes in the PBS group were significantly lower than those of the rDHN3-mF, rAd5-F, and rAd5-VP2-F2A-F groups ( p < 0.001). The SI values of peripheral blood lymphocytes in the rAd5-EGFP group were significantly lower than those in the rDHN3-mF, rAd5-F, and rAd5-VP2-F2A-F groups ( p < 0.01). However, there was no significant difference between the rDHN3-mF, rAd5-F, and rAd5-VP2-F2A-F groups ( p > 0.05), and no significant differences between the PBS and rAd5-EGFP groups ( p > 0.05). Under inactivated NDV stimulation, the SI values of peripheral blood lymphocytes in the PBS and rAd5-EGFP groups were significantly lower than those in the rDHN3-mF, rAd5-F, and rAd5-VP2-F2A-F groups ( p < 0.0001). SI values in the rAd-VP2-F2A-F group were significantly different from those of the rDHN3-mF and rAd5-F groups ( p < 0.05); no significant differences between the rDHN3-mF and rAd5-F groups ( p > 0.05), and there was no significant difference between the PBS and rAd5-EGFP groups ( p > 0.05) (ns—non-significant; * p < 0.05); ( B ) Statistical analysis of the peripheral blood lymphocyte stimulation index in IBD groups. From ( D ) above, it can be seen that the SI values of peripheral blood lymphocytes in the HVT-VP2 vector vaccines group, rAd5-VP2 group, and rAd5-VP2-F2A-F group were not significantly different from each other regardless of ConA stimulation or inactivated IBDV stimulation in the IBD group ( p > 0.05). However, the SI values of the above three groups were significantly higher than those of the rAd5- EGFP and the PBS groups ( p < 0.05). There was no significant difference between the PBS and rAd5-EGFP groups ( p > 0.05) (ns—non-significant); ( C , D ) Statistical analysis of the percentage of CD4+ and CD8+ T lymphocytes in the peripheral blood in ND groups. It can be seen from these two pictures that the percentages of CD4+ and CD8+ T lymphocytes in the rAd5-F, rDHN3-mF, and rAd5-VP2-F2A-F groups were significantly higher than those in the PBS group ( p < 0.05). There was no significant difference between the above three vaccine groups ( p > 0.05); ( E , F ) Statistical analysis of the percentage of CD4+ and CD8+ T lymphocytes in the peripheral blood in the IBD groups. It can be seen from these two pictures that the percentage of CD4+ and CD8+ T lymphocytes in the rAd5-VP2 group, the HVT-VP2 vector vaccines, and rAd5-VP2-F2A-F groups were significantly higher than in the PBS group ( p < 0.05). There was no significant difference between the three vaccine groups ( p > 0.05).

Article Snippet: The T-lymphocyte subpopulations were analyzed by flow cytometry (Beckman Coulter, Carlsbad, CA, USA) with the following antibodies: mouse anti-chicken CD3, mouse anti-chicken CD4, and mouse anti-chicken CD8α (Southern Biotech, Birmingham, AL, USA).

Techniques: Comparison, Plasmid Preparation, Vaccines

Journal: International Journal of Nanomedicine

Article Title: Surface engineered polyanhydride-based oral Salmonella subunit nanovaccine for poultry

doi: 10.2147/IJN.S185588

Figure Lengend Snippet: OMPs-specific cell-mediated immune response in OMPs-F-PNPs orally inoculated and Salmonella -challenged chickens. Notes: ( A ) Flow cytometry analyses of CD8 + /CD4 + cell ratio. Splenocytes were immunostained with fluorochrome-labeled mouse anti-chicken CD4 and mouse anti-chicken CD8α antibody. The frequency of CD4 + and CD8 + lymphocytes in the spleen was examined, and the result was expressed as CD8 + /CD4 + cell ratio. ( B ) Serum IFN-γ levels estimated by ELISA. ( C ) OMPs-specific lymphocytes’ proliferation was measured as stimulation index values in PBMCs by using a calorimetric assay. Each bar is the mean ± SEM of 8–10 chickens, and the data were analyzed by nonparametric Kruskal–Wallis test followed by P -value differences in between the groups determined by Mann–Whitney test. Asterisk refers to statistical difference between two indicated groups ( * P <0.05). OMPs-F-PNPs, OMPs and F-protein-entrapped and surface F-protein-coated PNPs. Abbreviations: Ch, challenge; F, flagellar; OMPs, outer membrane proteins; IFN-γ, interferon gamma; PBMCs, peripheral blood mononuclear cells; PNPs, polyanhydride nanoparticles; SEM, standard error of the mean.

Article Snippet: Splenocytes (1×10 6 cells/well) were seeded in 96-well plate and incubated with pretitrated 1:200 dilution of fluorescein isothiocyanate-conjugated mouse anti-chicken CD4 (Southern Biotech, Birmingham, AL, USA), pretitrated 1:400 dilution of phycoerythrin-conjugated mouse anti-chicken CD8α (Southern Biotech), or 1:100 diluted mouse IgG specific isotype control antibody for 20 minutes at 4°C.

Techniques: Flow Cytometry, Labeling, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Membrane

Journal: Helminthologia

Article Title: The Influence of Chestnut Wood and Flubendazole on Morphology of Small Intestine and Lymphocytes of Peripheral Blood, Spleen and Jejunum in Broiler Chickens

doi: 10.2478/helm-2019-0029

Figure Lengend Snippet: Subpopulations of lymphocytes in the peripheral blood (total counts = G.L –1 ) and spleen (relative percentage)(mean ± SD).

Article Snippet: Unlabelled primary mouse anti-chicken monoclonal antibodies (Southern Biotech, USA) for staining CD3 (CD3-UNLB, Clone CT-3, Cat. No. 8200-01), CD4 (CD4-UNLB, Clone CT-4, Cat. No. 8210-01), CD8 (CD8α-UNLB, Clone CT-8, Cat. No. 8220-01),IgA (IgA-UNLB, Clone A-1, Cat. No.8330-01), IgM (IgM-UN-LB, Clone M-1, Cat. No. 8300-01) were used in 1:10 dilution with PBS.

Techniques:

Journal: Helminthologia

Article Title: The Influence of Chestnut Wood and Flubendazole on Morphology of Small Intestine and Lymphocytes of Peripheral Blood, Spleen and Jejunum in Broiler Chickens

doi: 10.2478/helm-2019-0029

Figure Lengend Snippet: Subpopulations of lymphocytes in jejunal mucosa (mm 2 ).

Article Snippet: Unlabelled primary mouse anti-chicken monoclonal antibodies (Southern Biotech, USA) for staining CD3 (CD3-UNLB, Clone CT-3, Cat. No. 8200-01), CD4 (CD4-UNLB, Clone CT-4, Cat. No. 8210-01), CD8 (CD8α-UNLB, Clone CT-8, Cat. No. 8220-01),IgA (IgA-UNLB, Clone A-1, Cat. No.8330-01), IgM (IgM-UN-LB, Clone M-1, Cat. No. 8300-01) were used in 1:10 dilution with PBS.

Techniques: